Glycosaminoglycan composition of the vocal fold lamina propria in relation to function. The Annals of otology, rhinology, and laryngology [Ann Otol Rhinol Laryngol] Journal article | | Title | Glycosaminoglycan composition of the vocal fold lamina propria in relation to function. | | Author(s) | Hahn MS, Jao CY, Faquin W, Grande-Allen KJ | | Institution | Department of Chemical Engineering, Texas A&M University, College Station, TX, USA. | | Source | Ann Otol Rhinol Laryngol 2008 May; 117(5):371-81. | | MeSH | Animals
Biological Markers
Cicatrix
Disease Models, Animal
Dogs
Electrophoresis
Glycosaminoglycans
Humans
Hyaluronic Acid
Laryngeal Diseases
Prognosis
Respiratory Mucosa
Severity of Illness Index
Vocal Cords
| | Abstract | OBJECTIVES: This study was designed to quantify the specific glycosaminoglycans (GAGs) in the midmembranous vocal fold (VF) lamina propria (LP) and to interpret their presence in relation to the known stresses borne by each LP layer.
METHODS: GAGs from normal human LP and from both normal and scarred canine LPs were analyzed by fluorophore-assisted carbohydrate electrophoresis (FACE). Immunostaining was conducted to give insight into the spatial distribution of each GAG type.
RESULTS: Hyaluronan composes roughly 0.64% +/- 0.41% of the human LP as measured relative to tissue total protein. Chondroitin sulfate and/or dermatan sulfate (CS/DS), keratan sulfate, and heparan sulfate chains constitute approximately 23.9% +/- 12.1%, 14.7% +/- 6.1%, and 61.4% +/- 13.6%, respectively, of human LP sulfated GAGs.
CONCLUSIONS: Observed CS/DS sulfation patterns imply that versican is a major contributor to human LP CS levels. In addition, examination of LP GAG with respect to gender revealed a significant variation in total levels of CS/DS and a potential difference in the levels of versican relative to decorin and biglycan. In dogs, LP scarring appeared to result in a reduction in hyaluronan and CS/DS. These FACE results were combined with histologic data to update current descriptive models linking LP microstructure with the regional variations in LP loading. | | Language | eng | | Pub Type(s) | Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 18564535 |
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