| Title | Multiplex pathogen detection based on spatially addressable microarrays of barcoded resins. | | Author(s) | Blais DR, Alvarez-Puebla RA, Bravo-Vasquez JP, Fenniri H, Pezacki JP | | Institution | The Steacie Institute for Molecular Sciences, National Research Council of Canada, Ottawa, Canada. | | Source | Biotechnol J 2008 Jun 19. | | Abstract | Suspension microsphere immunoassays are rapidly gaining recognition in antigen identification and infectious disease biodetection due to their simplicity, versatility and high-throughput multiplex screening. We demonstrate a multiplex assay based on antibody-functionalized barcoded resins (BCRs) to identify pathogen antigens in complex biological fluids. The binding event of a particular antibody on given bead (fluorescence) and the identification of the specific pathogen agent (vibrational fingerprint of the bead) can be achieved in a dispersive Raman system by exciting the sample with two different laser lines. Anthrax protective antigen, Franciscella tularensis lipopolysaccharide and CD14 antigens were accurately identified and quantified in tetraplex assays with a detection limit of 1 ng/mL. The rapid, versatile and simple analysis enabled by the BCRs demonstrates their potential for multiplex antigen detection and identification in a reconfigurable microarray format. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18566958 |
|
