Isolation, culture and characterization of endothelial cells from human hypertrophic scar. Endothelium : journal of endothelial cell research [Endothelium] Journal article | | Title | Isolation, culture and characterization of endothelial cells from human hypertrophic scar. | | Author(s) | Wang XQ, Liu YK, Mao ZG, Qing C, Lu SL, Xu DZ | | Institution | Institute of Burn, Ruijin Hospital, Jiaotong University Medical School, Shanghai, The People's Republic of China. | | Source | Endothelium 2008 May-Jun; 15(3):113-9. | | Abstract | Although the etiology underlying scar formation is not well understood, previous studies revealed that endothelial cells play a role in the pathogenesis of scar development. Recently, the authors developed a reliable technique to obtain endothelial cells from hypertrophic scars that involved separation of cells from the scar tissue matrix and isolation from other cell types. Using phase-contract and electron microscopy, the cells were observed to have a characteristic morphology consistent with cells of endothelial origin. The cells were further characterized as endothelial cells by assessment of endothelin (ET)-1 and intercellular adhesion molecule (ICAM) mRNA expression, and the presence of factor VIII antigen, CD34, CD31, and VE-cadherin. This isolation method provides a simple method for culturing endothelial cells from hypertrophic scar tissue and should prove useful for studying the role of endothelial cell involvement in scar formation. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 18568951 |
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