| Title | Early expression of interleukin-2 mRNA by peripheral blood mononuclear cells isolated from nickel-allergic subjects and subsequently exposed to nickel in vitro. |
| Author(s) | Falsafi-Amin H, Holst M, Lundeberg L |
| Institution | Unit of Dermatology and Venereology, Department of Medicine. |
| Source | Immunopharmacol Immunotoxicol 2008; 30(2):227-34. |
| Abstract | The aim pf the present study was to characterize the time course of the synthesis of mRNA encoding interleukin-2 (IL-2) by peripheral blood mononuclear cells (PBMCs) isolated from nickel-allergic women and subsequently exposed to nickel sulphate in vitro. The level of this mRNA (determined by employing real-time polymerase chain reaction [PCR]) and of the corresponding protein (quantitated using an enzyme-linked immunosorbent assay [ELISA]) were significantly increased after only 10 and 24 h of incubation, respectively. The increased level of mRNA coding for IL-2 supports the hypothesis that this process is a key part of the response to nickel stimulation of PBMCs of hypersensitive subjects. |
| Language | eng |
| Pub Type(s) | Journal Article
|
| PubMed ID | 18569080 |
