| Title | Optimization of Fermentation Medium and Conditions for Mycelial Growth and Water-soluble Exo-polysaccharides Production by Isaria farinosa B05. | | Author(s) | Wang P, Jiang X, Jiang Y, Hu X, Hwang H | | Institution | College of Food Science and Engineering, Ocean University of China, Qingdao, P. R. China. | | Source | Prep Biochem Biotechnol 2008; 38(3):294-307. | | Abstract | The optimal fermentation medium and conditions for mycelial growth and water-soluble exo-polysaccharides production by Isaria farinosa B05 were investigated. The medium components and fermentation conditions were optimized according to the one at a time method, while the concentration of medium components was determined by the orthogonal matrix method. The results showed that the optimal fermentation medium was as follows: sucrose 3.5% (w/v), peptone 0.5%, yeast extract 0.2%, K(2)HPO(4) 0.1%, and MgSO(4) 0.05%. The suitable fermentation conditions were as follows: initial pH 7.0, temperature 25 degrees C, medium volume 75 mL/250 mL, inoculum volume 5% (v/v), time 5d. In such optimal nutrition and environmental conditions, the maximal mycelial yield was 2.124 g/100 mL after 4 day's fermentation, while maximal water-soluble exo-polysaccharides production reached 2.144 g/L after 5 day's fermentation. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 18569876 |
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