| Title | [Establishment of an in vivo model for duck hepatitis B virus infection using Hubei duckling.] | | Author(s) | Hu Q, Fang Y, Zhang ZM, Zhang YY, Zhang ZH, Yang DL | | Institution | Wuhan Centre for Disease Prevention and Control, Wuhan 430022, China. Corresponding author: Yang Dong-liang, E-mail: dlyang@tjh.tjmu.edu.cn, Tel: 027-83662894. | | Source | Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2008 Jun; 22(2):113-5. | | Abstract | OBJECTIVE: To develop a standard duck hepatitis B virus (DHBV) animal model using a local Hubei species of duck, Ma Ya, and use it as an in vivo experimental system to study antiviral strategies against hepatitis B.
METHODS: Two-day-old Ma Ya ducklings were experimentally infected via intraperitoneal injection with the DHBV inocula which was collected from the transfected culture supernatant of 1.5-fold-overlength genome recombinant plasmid. Blood samples were taken twice or thrice a week during post-inoculation for 50 days. Viremia was quantified by serum real-time PCR to show the peak. Antiviral treatment of the DHBV-infected ducklings was started 3 d post-inoculation. The animals received oral administration of lamivudine (3TC) at a dose of 25 mg/kg/d for 5 d, followed by a maintenance therapy thrice weekly for 3 more weeks. Serum was quantified to show the viremia peak and liver biopsy specimens were analysed by Southern blotting and in-situ hybridization at the end of antiviral drug treatment.
RESULTS: The experimental infection rate of 2-day-old ducklings was 87.5%. Viremia started to be detectable on day 7 and reached a peak on day 11 post-inoculation, followed by a decrease and fluctuations. Four weeks of oral administration of 3TC led to a significant decrease in viremia peak during. This effect was not sustained, as a rebound in viremia was observed after drug withdrawal. Similarly, the analysis of liver biopsies at the end of 3TC treatment showed a marked decrease in DHBV DNA. However, after drug withdrawal a rebound of intrahepatic DHBV DNA was observed in duck livers.
CONCLUSION: The Hubei duck model with experimental DHBV infection of transfected supernatant is more suitable for the hepadnavirus biologic research due to its stability and practicability. [Key words] Hepatitis B Virus; Models, animal; Genes, viral. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 18574531 |
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