| Title | Quantitative Survival of Aerobic and Anaerobic Microorganisms in Port-A-CulTM and Copan Transport Systems. | | Author(s) | Stoner KA, Rabe LK, Austin MN, Meyn LA, Hillier SL | | Institution | Magee-Womens Research Institute, Pittsburgh, PA; University of Pittsburgh, Department of Obstetrics, Gynecology and Reproductive Sciences, Pittsburgh, PA. | | Source | J Clin Microbiol 2008 Jun 25. | | Abstract | Transport media should preserve the viability and stability of microorganisms in clinical specimens. Port-A-Cul(TM) and Copan without charcoal transporters, both designed to preserve anaerobes, were evaluated. Dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. Combination I contained Candida albicans, Escherichia coli, Enterococcus spp., group B Streptococcus, Lactobacillus crispatus, and Staphylococcus aureus. Combination II contained Lactobacillus iners, Peptoniphilus asaccharolyticus, Mycoplasma hominis, Prevotella bivia, Prevotella corporis, Porphyromonas asaccharolytica, Mobiluncus curtisii, Peptostreptococcus anaerobius, and Gardnerella vaginalis. Duplicate swabs were placed in the two transporters and held for 24, 48, 72, and 96 hours at 4 degrees C and 24 degrees C. Both transporters maintained viability of organisms better at 4 degrees C than at 24 degrees C. P. bivia and P. corporis had loss of viability in both transporters at both temperatures. However, at 24 degrees C there was a significantly greater loss of organism viability when stored in Copan vs. Port-A-Cul(TM) transport media for M. hominis, P. bivia, P. corporis, and P. asaccharolyticus over 96 hours (P </= 0.002). Some organisms proliferated in transport media, but when transporters were held at 24 degrees C over 96 hours there was a significantly greater increase observed in the organism concentrations of group B Streptococcus, C. albicans, E. coli, and Enterococcus spp. in Copan vs. Port-A-Cul(TM) (P </= 0.002). At room temperature, Port-A-Cul(TM) is superior to Copan with respect to preservation of fastidious microorganisms and preventing proliferation of facultative organisms. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18579722 |
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