Ma C, Tang Z, Huo X, Yang X, Li W, Tan W
Real-time monitoring of double-stranded DNA cleavage using molecular beacons. [Journal Article, Research Support, Non-U.S. Gov't]
Talanta 2008 Jul 15; 76(2):458-61.
Traditional methods to assay enzymatic cleavage of DNA are discontinuous, time-consuming and laborious. Here, we report a new approach for real-time monitoring of double-stranded DNA cleavage by restriction endonuclease based on nucleic acid ligation using molecular beacon. Upon cleavage of DNA, the cleavage product can be ligated by DNA ligase, which results in a fluorescence enhancement of the molecular beacon. This method permits real-time monitoring of DNA cleavage and makes it easy to characterize the activity of restriction endonuclease and to study the cleavage reaction kinetics.
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