| Title | Real-time monitoring of double-stranded DNA cleavage using molecular beacons. | | Author(s) | Ma C, Tang Z, Huo X, Yang X, Li W, Tan W | | Institution | State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Yuelu-Qu District, Changsha 410082, Hunan, PR China. | | Source | Talanta 2008 Jul 15; 76(2):458-61. | | Abstract | Traditional methods to assay enzymatic cleavage of DNA are discontinuous, time-consuming and laborious. Here, we report a new approach for real-time monitoring of double-stranded DNA cleavage by restriction endonuclease based on nucleic acid ligation using molecular beacon. Upon cleavage of DNA, the cleavage product can be ligated by DNA ligase, which results in a fluorescence enhancement of the molecular beacon. This method permits real-time monitoring of DNA cleavage and makes it easy to characterize the activity of restriction endonuclease and to study the cleavage reaction kinetics. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 18585306 |
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