Kaldhone P, Nayak R, Lynne AM, David DE, McDermott PF, Logue CM, Foley SL
Characterization of Salmonella enterica serovar Heidelberg from Turkey-Associated Sources. [JOURNAL ARTICLE]
Appl Environ Microbiol 2008 Jun 27.
Salmonella enterica serovar Heidelberg strains are frequently associated with foodborne illness, with recent isolates showing higher rates of resistance to multiple antimicrobial agents. One hundred eighty S. enterica serovar Heidelberg isolates, collected from turkey-associated production and processing sources, were tested for antimicrobial susceptibility, and compared by pulsed field gel electrophoresis (PFGE) and plasmid profile analysis. The potential for the transfer of resistance between strains was studied by conjugation experiments. XbaI-digested PFGE analysis identified eight clusters (based on an 90% similarity), with the largest containing 71% of the isolates. Forty-two percent of the isolates were resistant to at least one of the 15 antimicrobial agents tested and 4% of the isolates were resistant to eight or more antimicrobial agents. Resistance was most commonly detected to streptomycin (32%), tetracycline (30%) and kanamycin (24%). Interestingly, the XbaI PFGE profiles of selective multidrug resistant strains (n=22) of S. enterica serovar Heidelberg from turkey-associated sources were indistinguishable from the predominant profile (JF6X01.0022), detected in isolates associated with human infections. These isolates were further differentiated into seven distinct profiles following digestion with BlnI enzyme, with the largest cluster compromising of 15 isolates from veterinary diagnostic and turkey processing environments. Conjugation experiments indicated that resistance to multiple antimicrobial agents was transferable among strains with diverse PFGE profiles.
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