| Title | Quantitative analysis of rabeprazole sodium in commercial dosage forms by spectrophotometry. | | Author(s) | Rahman N, Bano Z, Azmi SN | | Institution | Department of Chemistry, Aligarh Muslim University. | | Source | Chem Pharm Bull (Tokyo) 2008 Jul; 56(7):995-1001. | | Abstract | The main aim of this work is to develop and validate two spectrophotometric methods for the quantitative analysis of rabeprazole sodium in commercial dosage forms. Method A is based on the reaction of drug with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) in the presence of ammonium cerium(IV) nitrate in acetic acid medium at room temperature to form red-brown product which absorbs maximally at 470 nm. Method B utilizes the reaction of rabeprazole sodium with 1-chloro-2,4-dinitrobenzene (CDNB) in dimethyl sulfoxide (DMSO) at 45+/-1 degrees C to form yellow colored Meisenheimer complex. The colored complex has a characteristic band peaking at 420 nm. Under the optimized reaction conditions, proposed methods are validated as per ICH guidelines. Beer's law is obeyed in the concentration ranges of 14-140 and 7.5-165 mug ml(-1) with linear regression equations of A=6.041x10(-4)+1.07x10(-2)C and A=1.020x10(-3)+5.0x10(-3)C for methods A and B, respectively. The limits of detection for methods A and B are 1.38 and 0.75 mug ml(-1), respectively. Both methods have been applied successfully for the estimation of rabeprazole sodium in commercial dosage forms. The results are compared with the reference UV spectrophotometric method. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 18591818 |
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