Muñoz R, Carvajal C, Escalona A, Boza C, Pérez G, Ibáñez L, Fardella C
11beta-Hydroxysteroid Dehydrogenase Type 1 is Overexpressed in Subcutaneous Adipose Tissue of Morbidly Obese Patients. [JOURNAL ARTICLE]
Obes Surg 2008 Jul 1.
BACKGROUND: 11beta-Hydroxysteroid dehydrogenase type 1 (11beta-HSD1) enzyme catalyzes interconversion of inactive cortisone to active cortisol. Its expression in adipose tissue has been associated with obesity and some of its metabolic disorders. Controversies regarding which fat depots [subcutaneous adipose tissue (SAT) or visceral adipose tissue (VAT)] have higher expression still remain. The aim of this work was to evaluate 11beta-HSD1 expression in SAT and VAT of obese patients and evaluate its association to metabolic features of metabolic syndrome.
METHODS: In 32 morbidly obese patients, paired samples of SAT and VAT were collected. All patients, 40.2 +/- 12.3 years and 36.7 +/- 3.8 body mass index (BMI), underwent sleeve gastrectomy or laparoscopic gastric bypass. Gene expression of 11beta-HSD1 in adipose tissue samples were determined by real-time reverse transcriptase polymerase chain reaction. Spearman correlation test was used to evaluate relationships between 11beta-HSD1 levels and clinical and biochemical parameters.
RESULTS: 11beta-HSD1 mRNA levels were higher in SAT than in VAT, with median expression levels of 11.4 arbitrary units (AU) and 7.8 AU, respectively (p = 0.03). SAT 11beta-HSD1 mRNA were correlated with VAT mRNA levels (r = -0.6, p = 0.018) and hip circumference (r = 0.66, p = 0.018). SAT 11beta-HSD1 levels increase parallel according to BMI category. We did not find a correlation between SAT or VAT with fasting glucose (r = 0.15, p = NS), total cholesterol (r = 0.13, p = NS), triglycerides (r = 0.04, p = NS), and high-density lipoprotein (r = -0.16, p = NS). However, SAT expression in patients with features of MS was higher than those without features of MS.
CONCLUSIONS: Our results demonstrate that SATs express higher 11beta-HSD1 mRNA levels than VAT. This finding highlights the importance of SAT in obesity and its possible role on metabolic disorders associated with obesity.
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