| Title | Molecular cloning, characterization and evolutionary analysis of estrogen receptors from phylogenetically ancient fish. | | Author(s) | Katsu Y, Kohno S, Hyodo S, Ijiri S, Adachi S, Hara A, Guillette LJ, Iguchi T | | Institution | Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, 5-1 Higashiyama, Myodaiji, Okazaki 444-8787, Japan; Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies, Okazaki 444-8787, Japan; Department of Zoology, 223 Bartram Hall, Box 118525, University of Florida, Gainesville, FL 32611, USA; Laboratory of Physiology, Ocean Research Institute, University of Tokyo, Nakano, Tokyo 164-8639, Japan; Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan. | | Source | Endocrinology 2008 Jul 17. | | Abstract | Estrogens are necessary for ovarian differentiation during a critical developmental stage in many vertebrates and they promote the growth and differentiation of the adult female reproductive system. To understand the evolution of vertebrate estrogen receptors and to evaluate estrogen receptor-ligand interactions in phylogenetically ancient fish, we used PCR techniques to isolate the cDNA encoding estrogen receptors (ESRs) from lungfish, sturgeon and gar. Sequence analyses indicate that these fishes have two estrogen receptors, ESR1 (ERalpha) and ESR2 (ERbeta) as previously reported for other vertebrate species, but a second type of ESR2 (ERbeta2) was not found as has been reported in a number of teleost fishes. Phylogenetic analysis of the ESR sequences indicated that the lungfish ESRs are classified to the tetrapod ESR group, not with the teleost fish ESRs as are the ESRs from gar and sturgeon. Using transient transfection assays of mammalian cells, ESR proteins from these three ancient fishes displayed estrogen-dependent activation of transcription from an estrogen-responsive-element containing promoter. We also examined the estrogenic potential of o,p'-DDT and p,p'-DDT as well as one of its common metabolites, p,p'-DDE on the ESRs from these fishes. Lungfish ESR1 was less sensitive to DDT/DDE than the ESR1 from the other two fishes. The response of lungfish ESR1 to these pesticides is similar to the pattern obtained from salamander ESR1. These data provide a basic tool allowing future studies examining the receptor-ligand interactions and endocrine disrupting mechanisms in three species of phylogenetically ancient fish and also expands our knowledge of estrogen receptor evolution. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18635653 |
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