| Title | Ligand-induced Flt3-downregulation modulates cell death associated proteins and enhances chemosensitivity to idarubicin in THP-1 acute myeloid leukemia cells. | | Author(s) | Oveland E, Gjertsen BT, Wergeland L, Selheim F, Fladmark KE, Hovland R | | Institution | Proteomic Unit at University of Bergen, Bergen, Norway; Department of Biomedicine, University of Bergen, Bergen, Norway. | | Source | Leuk Res 2008 Aug 6. | | Abstract | Sustained ligand stimulation of the receptor tyrosine kinase Flt3 resulted in its downregulation and a refractory signaling phase in primary acute myeloid leukemia (AML) cells and in the AML cell line THP-1. Stable isotope amino acid labeling in cell culture and mass spectrometry were used to compare protein expression patterns in THP-1 before and after Flt3-downregulation. 375 distinct proteins were identified where ATP-dependent RNA helicase DDX3, HNRPU, Matrin-3, Importin-7 and Bax were among the 25 most upregulated proteins and Hausp/UBP7, UBE2N and ERp29 among the 17 most downregulated. THP-1 cells with receptor downregulation were sensitized to idarubicin-induced apoptosis but not cytarabine. We hypothesize that FL-induced receptor modulation may chemosensitize selected AML subsets. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18691757 |
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