| Title | The Survival of Herpes Simplex Virus in Preserved Murine Corneas. | | Author(s) | Robert PY, Ducher V, Pleyer U, Denis F, Adenis JP, Ranger-Rogez S | | Institution | Department of Ophthalmology, EA 3175, CHU Dupuytren, Limoges, France. | | Source | Ophthalmic Res 2008 Aug 12; 40(6):329-332. | | Abstract | Background/Aims: This study investigates the survival of HSV in infected mouse corneas, in the conditions of normal human eye bank preservation.
Methods: Hundred seventy-two BALB/C mice infected with herpes simplex virus 1 (HSV-1) (KOS) were randomly assigned to either: no preservation in group 1 (n = 62), 31 degrees C preservation for 3 weeks in group 2 (n = 70) or 4 degrees C preservation for 8 days in group 3 (n = 40). The presence of HSV-1 was thereafter detected by viral culture and PCR.
Results: In groups 1, 2 and 3, HSV-1 was detected by culture in 22 (35.5%), 1 (1.4%) and 0 (0.0%) of the corneas, and by PCR in 27 (43.7%), 3 (4.2%) and 7 (17.5%) of the corneas respectively. When compared to group 1, HSV was detected significantly less often in groups 2 (p < 0.0001) and 3 (p < 0.0001).
Conclusions: HSV-1 DNA undergoes a degradation during corneal preservation. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18698148 |
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