| Title | Oxidative Effects of Gemfibrozil on Anion Influx and Metabolism in Normal and Beta-Thalassemic Erythrocytes: Physiological Implications. | | Author(s) | Tellone E, Ficarra S, Giardina B, Scatena R, Russo A, Clementi ME, Misiti F, Bellocco E, Galtieri A | | Institution | Organic and Biological Chemistry Department, University of Messina, Salita Sperone 31, 98166, Messina, Italy. | | Source | J Membr Biol 2008 Sep 29. | | Abstract | To further clarify some peculiar molecular mechanisms related to the physiology and pathophysiology of erythrocytes with respect to oxygen binding and release, metabolism and senescence, we investigated the oxidative effects of gemfibrozil in normal and beta-thalassemic red blood cells. Our results showed that the oxidative stress promoted by the drug, through a direct interaction with hemoglobin, may lead to activation of caspase 3, which in turn influences the band 3 anion flux and glucose metabolism. In a comparative context, we also evaluated the effect on band 3 and caspase 3 activation of orthovanadate (a phosphatase inhibitor) and t-butylhydroperoxide (a known oxidant). The results support the hypothesis that gemfibrozil influences band 3 function through several mechanisms of action, centered on oxidative stress, which induces significant alterations of glucose metabolism. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18821038 |
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