| Title | 9-cis retinoic acid induces retinoid X receptor localized to the mitochondria for mediation of mitochondrial transcription. | | Author(s) | Lin YW, Lien LM, Yeh TS, Wu HM, Liu YL, Hsieh RH | | Institution | Department of Urology, Taipei Medical University-Wan Fang Hospital. | | Source | Biochem Biophys Res Commun 2008 Oct 3. | | Abstract | We previously reported that 9-cis retinoic acid (RA) treatment induced an increase in mitochondrial (mt)DNA transcription. In order to extend these results, we tested various concentrations of 9-cis RA were used to treat 143B cells. Cells with low membrane potential treated with 9-cis RA showed significantly lower amounts of RXRalpha in mitochondria. We also found lower RXRalpha levels in mtDNA-depleted cells. Treating cells with 9-cis RA significantly increased expression of ND1, ND6, and COX I RNA. However, 9-cis RA-treatment did not appear to induce any significant changes in mtDNA copy number or mitochondrial mass. This study represents that 9-cis RA increases mtDNA transcription but not mtDNA replication, and it suggests that the effects of 9-cis RA on mitochondria are mediated by RXR localization to mitochondria. In addition, this is the first report that 9-cis RA regulation of RXR mitochondrial translocation depends on mitochondrial membrane potential and ATP. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18840407 |
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