| Title | Functional expression of the glycine transporter 1 on bullfrog retinal cones. | | Author(s) | Zhang XJ, Ge LH, Liu J, Yang XL | | Institution | Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China. | | Source | Neuroreport 2008 Nov 19; 19(17):1667-71. | | MeSH | Amoxapine
Animals
Glycine
Glycine Agents
Glycine Plasma Membrane Transport Proteins
Male
Membrane Potentials
Neurotransmitter Uptake Inhibitors
Patch-Clamp Techniques
Rana catesbeiana
Retinal Cone Photoreceptor Cells
Sarcosine
Strychnine
| | Abstract | Using patch clamp techniques, we characterized glycine-induced currents from cones in bullfrog retinal slices. Application of glycine to cone terminals induced an inward current, which was in part suppressed by strychnine. The remaining strychnine-resistant current component, which did not show polarity reversion in a range of -120 mV to +40 mV, was blocked by N[3-(4'-fluorophenyl)-3-(4'-phenylphenoxy)propyl] sarcosine, an antagonist of glycine transporter 1 (GlyT1), but not affected by amoxapine, an inhibitor of glycine transporter 2. Application of sarcosine, an agonist of GlyT1, to cone terminals induced an inward current that was completely suppressed by N[3-(4'-fluorophenyl)-3-(4'-phenylphenoxy)propyl] sarcosine or when external Na in Ringer's was replaced by choline. All these results show for the first time the functional expression of GlyT1 on bullfrog cones. | | Language | eng | | Pub Type(s) | In Vitro
Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 18841092 |
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