| Title | Physiologic and pathologic levels of reactive oxygen species in neat semen of infertile men. | | Author(s) | Desai N, Sharma R, Makker K, Sabanegh E, Agarwal A | | Institution | Center for Reproductive Medicine, Glickman Urological and Kidney Institute and Department of Obstetrics and Gynecology and Women's Health Institute, Cleveland Clinic, Cleveland, Ohio. | | Source | Fertil Steril 2008 Oct 18. | | Abstract | OBJECTIVE: To define physiologic levels of reactive oxygen species in infertile men and establish a cutoff value of reactive oxygen species level in neat semen with a high sensitivity and specificity to differentiate infertile men from fertile donors (controls).
DESIGN: Reactive oxygen species levels were measured in the neat semen samples (n = 51) from fertile donors and infertile patients (n = 54).
SETTING: Reproductive research laboratory at a tertiary care hospital.
PATIENT(S): Infertile patients from male infertility clinic.
INTERVENTION(S): Reactive oxygen species measurement in neat semen sample using luminol-based chemiluminescence method, receiver operating characteristic curves.
MAIN OUTCOME MEASURE(S): Seminal reactive oxygen species levels, cutoff value, sensitivity and specificity, positive and negative predictive values.
RESULT(S): The best cutoff value to distinguish between healthy fertile donors and infertile men was 0.0185 x 10(6) counted photons per minute/20 x 10(6) sperm. At this threshold, the specificity was 82% and the sensitivity was 78%. This value can be defined as basal reactive oxygen species level in infertile men.
CONCLUSION(S): Reactive oxygen species levels in neat semen samples as measured by luminol-based chemiluminescence are a highly specific and sensitive test in the diagnosis of infertility. This test also may help clinicians treat patients with seminal oxidative stress. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 18937945 |
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