Quantification of isradipine in human plasma using LC-MS/MS for pharmacokinetic and bioequivalence study. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | Quantification of isradipine in human plasma using LC-MS/MS for pharmacokinetic and bioequivalence study. | | Author(s) | Park JH, Park YS, Rhim SY, Jhee OH, Kim SH, Yang SC, Lee MH, Shaw LM, Kang JS | | Institution | Department of Pharmacology & Institute of Biomedical Sciences, College of Medicine; Department of Bioengineering, Hanyang University, Seoul 133-791, South Korea. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2008 Nov 18. | | Abstract | A highly sensitive and rapid method for the analysis of isradipine in human plasma using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed. The procedure involves a simple liquid-liquid extraction of isradipine and amlodipine (IS, internal standard) with methyl-t-butyl ether after alkaline treatment and separation by RP-HPLC. Detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 372.1-->m/z 312.2 and m/z 408.8-->m/z 237.9, for quantification of isradipine and IS, respectively. The standard calibration curves showed good linearity within the range of 10 to 5000pg/mL (r(2)>/=0.9998). The lower limit of quantitation (LLOQ) was 10pg/mL. The retention times of isradipine (0.81min) and IS (0.65min) suggested the potential for high throughput of the proposed method. In addition, no significant metabolic compounds were found to interfere with the analysis. This method offered good precision and accuracy and was successfully applied for the pharmacokinetic and bioequivalence studies of 5mg of sustained-release isradipine in 24 healthy Korean volunteers. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19041284 |
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