Ohyabu Y, Kaul Z, Yoshioka T, Inoue K, Sakai S, Mishima H, Uemura T, Wadhwa R, Kaul SC
Stable and Non-Disruptive In Vitro/In Vivo Labeling of Mesenchymal Stem Cells by Internalizing Quantum Dots. [JOURNAL ARTICLE]
Hum Gene Ther 2008 Dec 1.
Recent progress in the stem cell research has prioritized the refinement of cell labeling techniques for in vitro and in vivo basic and therapeutic studies. Although quantum dots, because of their optical properties, are emerging as favorable nanoparticles for bio-imaging, substantial refinements or modifications that would improve their bio-compatiblilty are still required. We report here that the internalizing Quantum dots (i-QDs) generated by their conjugation with an internalizing antibody against an hsp70 family stress chaperone, mortalin, offered an efficient, genetically noninvasive, non toxic and functionally inert way to label mesenchymal stem cells (MSCs). The i-QD labeled MSCs underwent normal adipocyte, osteocyte, and chondrocyte differentiation in vitro and in vivo suggesting a desired application of i-QDs in in vivo diagnostics, regenerative and therapeutic medicine.
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