| Title | Effects of sesquiterpene lactones on melanogenesis in mouse B16 melanoma cells. | | Author(s) | Ohguchi K, Ito M, Yokoyama K, Iinuma M, Itoh T, Nozawa Y, Akao Y | | Institution | Gifu International Institute of Biotechnology, Kakamigahara, Gifu, Japan. kohguchi@giib.or.jp | | Source | Biol Pharm Bull 2009 Feb; 32(2):308-10. | | MeSH | 1-Methyl-3-isobutylxanthine
Animals
Asteraceae
Blotting, Western
Cell Line, Tumor
Cell Survival
Lactones
Melanins
Melanoma, Experimental
Mice
Monophenol Monooxygenase
Phosphodiesterase Inhibitors
Plant Extracts
Plant Leaves
Reverse Transcriptase Polymerase Chain Reaction
Sesquiterpenes
Structure-Activity Relationship
Tanacetum
| | Abstract | In this study, we examined the effect of sesquiterpene lactones isolated from Calea urticifolia and Tanacetum parthenium (feverfew) on melanogenesis in mouse B16 melanoma cells. In response to 3-isobutyl-1-methylxanthin (IBMX), B16 melanoma cells underwent differentiation characterized by increased melanin biosynthesis. Treatment of sesquiterpene lactones at lower concentration (1 microM) significantly blocked IBMX-induced melanogenesis, but did not induce the inhibitory activity of cell growth. Among them, 2,3-epoxyjuanislamin exhibited a potent inhibitory effect on melanogenesis. Treatment of B16 cells with 2,3-epoxyjuanislamin elicited significant decreases in tyrosinase protein and mRNA levels. These results demonstrated that the inhibitory effects of sesquiterpene lactones on melanin biosynthesis may be due to the suppression of tyrosinase expression. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19182396 |
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