Determination of anabolic steroids in bovine urine by liquid chromatography-tandem mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | Determination of anabolic steroids in bovine urine by liquid chromatography-tandem mass spectrometry. | | Author(s) | Kaklamanos G, Theodoridis G, Dabalis T | | Institution | Veterinary Laboratory of Serres, Terma Omonoias, 62110, Serres, Greece. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2009 Mar 28. | | Abstract | A liquid chromatography-tandem mass spectrometric (LC-MS/MS) multi-method has been developed for the determination of 15 anabolic steroids in bovine urine (diethylstilbestrol, dienestrol, hexestrol, beta-estradiol, ethynylestradiol, alpha/beta-boldenone, alpha-nortestosterone, alpha/beta-zearalenol, alpha/beta-zaeralanol, zearalenone, stanozolol and 16beta-OH-stanozolol). The procedure involved enzymatic hydrolysis, extraction with tert-butyl methyl ether, a washing step with hexane and final clean-up with SPE with Oasis HLB and Amino cartridges. The analytes were quantified by liquid chromatography coupled to a tandem mass spectrometer (LC-TSQ Quantum AM) operating in both positive and negative atmospheric pressure chemical ionisation (APCI). Data acquisition was performed in multiple reaction monitoring (MRM) mode quantifying two diagnostic product ions from a chosen precursor. The method was validated according to the Commission Decision 2002/657/EC, for the detection and confirmation of residues in products of animal origin. The method specificity, sensitivity, accuracy and precision were evaluated. The decision limits CCalpha ranged from 0.06 to 0.26ng/ml and the detection capabilities CCbeta ranged from 0.11 to 0.49ng/ml. The developed method is sensitive and useful for detection, quantification and confirmation of these anabolic steroids in bovine urine and can be used for residue control programs. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19375394 |
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