| Title | Second-Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Fluphenazine Hydrochloride and Nortriptyline Hydrochloride in Pharmaceutical Preparations. | | Author(s) | Walash MI, El-Brashy A, El-Enany N, Kamel ME | | Institution | Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt. | | Source | J Fluoresc 2009 May 6. | | Abstract | A rapid, simple, and highly sensitive second-derivative synchronous fluorimetric (SDSF) method has been developed for the simultaneous analysis of binary mixtures of fluphenazine hydrochloride (FLZ) and nortriptyline hydrochloride (NTP) in their co-formulated tablets. The method is based upon measurement of the native fluorescence of these drugs at constant wavelength difference (Deltalambda) = 120 nm in acetic acid. The different experimental parameters affecting the fluorescence intensity of the studied drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the range of 0.25-3.0 and 1-10 mug/ml for FLZ and NTP respectively, with lower detection limits (LOD) of 0.05 and 0.18 mug/ml and quantitation limits of 0.15 and 0.53 mug/ml for FLZ and NTP respectively. The proposed method was successfully applied for the determination of the studied compounds in their synthetic mixtures and in commercial co-formulated tablets. The results obtained were in good agreement with those obtained by the reference methods. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19418209 |
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