Fluconazole alters CYP26 gene expression in mouse embryos. Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] Journal article | | Title | Fluconazole alters CYP26 gene expression in mouse embryos. | | Author(s) | Tiboni GM, Marotta F, Carletti E | | Institution | Sezione di Ostetricia e Ginecologia, Dipartimento di Medicina e Scienze dell'Invecchiamento, Facoltà di Medicina e Chirurgia, Università "G. d'Annunzio" di Chieti-Pescara, Italy. | | Source | Reprod Toxicol 2009 Feb; 27(2):199-202. | | Abstract | Disruption of embryonal retinoic acid homeostasis has been postulated to represent an etiological factor involved in the onset of fluconazole-induced teratogenesis. In the present study the impact of a teratogenic pulse of fluconazole on the gene expression of cytochrome P450 (CYP) 26 isoforms, which plays a central role in maintaining proper retinoic acid levels by mediating its degradation, was investigated. ICR pregnant mice were orally administered with 0 (vehicle) or 700mg/kg of fluconazole on gestation day 8. Embryos were collected 12, 24 and 48h after treatment. Quantitative real-time reverse-transcription polymerase chain reaction (quantitative real-time RT-PCR) assay was used to quantify the mRNA expression of CYP26a1, CYP26b1 and CYP26c1 in embryos. As result, fluconazole exposure was associated to an up-regulation of CYP26a1, CYP26b1, whereas no significant change was identified for the CYP26c1 isoform. This study demonstrates the capacity of fluconazole to alter CYP26 gene expression in mouse embryos. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19429397 |
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