| Title | The Nematode Resistance Allele at the rhg1 Locus Alters the Proteome and Primary Metabolism of Soybean Roots. | | Author(s) | Afzal AJ, Natarajan A, Saini N, Iqbal MJ, Geisler M, El Shemy HA, Mungur R, Willmitzer L, Lightfoot DA | | Institution | Department of Molecular Biology, Microbiology and Biochemistry, Southern Illinois University at Carbondale, IL, 62901, USA; Genomics Core Facility; Department of Plant Soil and Agricultural Systems, and Center for Excellence in Soybean Research, Teaching and Outreach, Southern Illinois University at Carbondale, Carbondale, IL 62901-4415, USA; Institute for Advanced Learning and Research , 150 Slayton Ave, Danville, VA 24540, USA; Department of Plant Biology, Southern Illinois University at Carbondale, IL, 62901, USA; Max Planck Institute for Molecular Plant Physiology, Am Muehlenberg 1, Potsdam 14476, Germany. | | Source | Plant Physiol 2009 May 8. | | Abstract | Heterodera glycines I., the cyst nematode (SCN), causes the most damaging chronic disease of soybean. Host resistance requires the resistance allele at rhg1. Resistance destroys the giant cells created in the plants roots by the nematodes about 24-48 h after commencement of feeding. In addition, 4-8 days later a systemic acquired resistance (SAR) develops that discourages later infestations. The molecular mechanisms that control the rhg1 mediated resistance response appear to be multigenic and complex judged by transcript abundance changes, even in near isogeneic lines (NILs). This study aimed to focus on key post-transcriptional changes by identifying proteins and metabolites that were increased in abundance in both resistant and susceptible NILs. Comparisons were made among NILs 10 days after SCN infestation and without SCN infestation. Two dimensional gel electrophoresis resolved more than 1,000 protein spots on each gel. Only thirty protein spots with a significant (P<0.05) difference in abundance of 1.5 fold or more were found among the four treatments. The proteins in these spots were picked, trypsin digested and analyzed using quadruple time-of-flight (Q-TOF) tandem mass spectrometry. Protein identifications could be made for 24 out of the 30 spots. Four spots contained two proteins so that 28 distinct proteins were identified. The proteins were grouped into six functional categories. Metabolite analysis by GC-MS identified 131 metabolites among which 58 were altered by one or more treatment, 28 were involved in primary metabolisms. Taken together the data showed seventeen pathways that were altered by rhg1 controlled metabolisms associated with SAR-like responses including xenobiotic, phytoalexin, ascorbate and inositol metabolism as well as primary metabolisms like amino acid metabolism and glycolysis. The pathways impacted by the rhg1 allelic state and SCN infestation agreed with transcript abundance analyses but identified a smaller set of key proteins. Six of the proteins lay within the same region of the interactome identifying a key set of 159 proteins. Finally, two proteins (glucose 6 phosphate isomerase, EC 5.3.1.9; and isoflavone reductase, EC 1.3.1.45) and two metabolites (maltose and unidentified) differed in resistant and susceptible NILs without SCN infestation and may form the basis of a new assay for the selection of resistance to SCN in soybean. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19429603 |
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