| Title | GnRH analogue remarkably down-regulates inflammatory proteins in peritoneal fluid proteome of women with endometriosis. | | Author(s) | Ferrero S, Gillott DJ, Remorgida V, Anserini P, Ragni N, Grudzinskas JG | | Institution | Reproductive Physiology Laboratory, St. Bartholomew's School of Medicine and Dentistry, Queen Mary and Westfield College, 48-53 St. Bartholomew's Close, St. Bartholomew's Hospital, West Smithfield, London, EC1A 7BE, United Kingdom. dr@simoneferrero.com | | Source | J Reprod Med 2009 Apr; 54(4):223-31. | | Abstract | OBJECTIVE: To determine the changes in the peritoneal fluid proteome of women with endometriosis determined by the administration of gonadotropin-releasing hormone analogues (GnRH-a).
STUDY DESIGN: Peritoneal fluid samples were collected during laparoscopy from patients under GnRH-a and from women who did not receive any type of hormonal treatment in the 6 months before surgery. Samples were subjected to 2-D gel electrophoresis and compared by computerized analysis. Protein spots differentially expressed between the study groups were identified by liquid chromatography tandem mass spectrometry.
RESULTS: More than 470 protein spots were analyzed. Several proteins with significant alterations were found. The down-regulated molecules were isoforms of alpha 2-HS glycoprotein, alpha 1-antitrypsin, S100-A8, haptoglobin alpha chain and vitamin D-binding protein. No protein spot had significantly higher expression in peritoneal fluid of women under GnRH-a than in untreated patients.
CONCLUSION: Several inflammatory molecules present in peritoneal fluid are down-regulated during treatment with GnRH-a; administration of this drug reduces the inflammation in the peritoneal cavity. | | Language | eng | | Pub Type(s) | Journal Article
| | PubMed ID | 19438164 |
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