| Title | Treatment of human cancer cells with selenite or tellurite in combination with auranofin enhances cell death due to redox shift. | | Author(s) | Rigobello MP, Gandin V, Folda A, Rundlöf AK, Fernandes AP, Bindoli A, Marzano C, Björnstedt M | | Institution | Dip. Chimica Biologica, Università di Padova, Viale G. Colombo 3, 35121 Padova, Italy. | | Source | Free Radic Biol Med 2009 May 29. | | Abstract | Selenium is an essential trace element incorporated as selenocysteine in 25 human selenoproteins. Among them are thioredoxin reductases (TrxR) and glutathione peroxidases (GPx) all central proteins in the regulation of cellular thiol redox state. In the present paper the effects of selenite and tellurite treatment in human cancer cells are reported and compared. Our results show that both selenite and tellurite, at relatively low concentrations, are able to increase the expression of mitochondrial and cytosolic TrxR in cisplatin sensitive (2008) and resistant (C13) phenotypes. We further investigated the cellular effects induced by selenite or tellurite in combination with the specific TrxR inhibitor, auranofin. Selenite pre-treatment induces a dramatic increase of auranofin cytotoxicity both in resistant and sensitive cells. Investigation of TrxR activity and expression levels as well as the cellular redox state demonstrates the involvement of TrxR inhibition and redox changes in selenite and auranofin combined action. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19486940 |
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