| Title | Caffeine augments Alprazolam induced cytotoxicity in human cell lines. | | Author(s) | Saha B, Mukherjee A, Samanta S, Saha P, Ghosh AK, Santra CR, Karmakar P | | Institution | Department of Life Science & Biotechnology, Jadavpur University, Kolkata - 700 032, W.B., India. | | Source | Toxicol In Vitro 2009 May 30. | | Abstract | Combined effects of alprazolam (Alp), a member of benzodiazepine group of drugs and caffeine on human cell lines, HeLa and THP1 were investigated in this study. Alp mediated cytotoxicity was enhanced while caffeine was present. The cell death was confirmed by observing morphological changes, LDH assay and membrane anisotropic study. Also such combined effects induced elevated level of ROS and depletion of GSH. The mechanism of cell death induced by simultaneous treatment of Alp and caffeine was associated with the calcium-mediated activation of mu-calpain, release of lysosomal protease cathepsin B, activation of PARP and cleavage of caspase 3. Our results indicate that, Alp alone induces apoptosis in human cells but in the presence of caffeine it augments necrosis in a well-regulated pathway. Thus our observations strongly suggest that, alprazolam and caffeine together produce severe cytotoxicity in human cell lines. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19490937 |
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