Frontal analysis of cell-membrane chromatography for determination of drug-alpha(1D) adrenergic receptor affinity. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | Frontal analysis of cell-membrane chromatography for determination of drug-alpha(1D) adrenergic receptor affinity. | | Author(s) | Zeng A, Yuan B, Wang C, Yang G, He L | | Institution | School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2009 May 18. | | Abstract | The aim of the present study was to determine drug-alpha(1D) adrenergic receptor (AR) affinity by frontal analysis of cell-membrane chromatography (CMC). The cell-membrane stationary phase (CMSP) was prepared by immobilizing rat aorta cell membranes on porous silica, and the resulting CMSP was used to determine drug binding affinity to alpha(1D)-AR by frontal analysis. The CMSP of rat aorta was stable and reproducible. Relative binding affinities (dissociation constant, K(d)) were determined by frontal chromatography for prazosin (166.13+/-18.36nmol), BMY7378 (537.40+/-30.84nmol), phentolamine (646.92+/-23.17nmol), 5-methylurapidil (725.66+/-25.48nmol), oxymetazoline (910.56+/-40.62nmol) and methoxamine (1299.27+/-51.73nmol). These results were consistent with the affinity rank order and showed a good correlation with the affinity of the same compounds for the cloned alpha(1D)-AR subtype obtained from radioligand-binding assay. The study demonstrates that frontal analysis of CMC may be used for direct determination of drug-receptor binding interactions, and that CMC is an alternative reliable method to quantitatively study ligand-receptor interactions. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19493707 |
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