| Title | Microwave sterilization of growth medium alleviates inhibition of Aggregatibacter actinomycetemcomitans by Maillard Reaction Products. | | Author(s) | Bhattacharjee MK, Sugawara K, Ayandeji OT | | Institution | Department of Chemistry and Biochemistry, Long Island University, Brooklyn, New York. | | Source | J Microbiol Methods 2009 Jun 11. | | Abstract | Aggregatibacter (Actinobacillus) actinomycetemcomitans, is the causative agent of localized aggressive periodontitis and endocarditis. The bacteria grow slowly even in a rich medium and rapidly lose viability after about 19 hours of growth. One of the reasons for the slow growth and for the decreased viability is the conventional method of making growth media by autoclaving. Faster growth and greater viability was observed in both broth and plates if the growth media were sterilized by microwave radiation rather than by autoclaving. One difference between autoclaved and microwaved media is that the autoclaved media are darker brown in color, which is known to be due to Maillard reaction products, also known as Amadori products. The Maillard reaction products formed by autoclaving a mixture of lysine and glucose was shown to inhibit growth of A. actinomycetemcomitans. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19524624 |
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