| Title | Changes in phenotype of monocyte-like THP-1 cells associated with transendothelial migration. |
| Author(s) | Starikova EA, Sokolov DI, Chernova AA, Freidlin IS |
| Institution | Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia. starickova@yandex.ru |
| Source | Bull Exp Biol Med 2008 Nov; 146(5):596-8. |
| MeSH | Antigens, CD11b
Antigens, CD14
Cell Line
Cell Movement
Endothelial Cells
HLA-DR Antigens
Humans
Interferon-gamma
Interleukin-4
Macrophages
Phenotype
Tumor Necrosis Factor-alpha
|
| Abstract | The level of expression of CD11b and HLA-DR surface molecules on monocyte-like THP-1 cells increased significantly as a result of transmigration of these cells through a monolayer of endothelial cells. The expression of all studied markers (CD11b, HLA-DR, and CD-14) increased significantly after transendothelial migration in the presence of TNF-alpha and IFN-gamma. The changes in surface phenotype of THP-1 cells after transendothelial migration in the presence of TNF-alpha were more pronounced than in the presence of IFN-gamma. Transendothelial migration of THP-1 cells in the presence of IL-4 caused less pronounced changes in the surface phenotype, which did not differ from changes in transmigration without cytokines. |
| Language | eng |
| Pub Type(s) | Journal Article
Research Support, Non-U.S. Gov't
|
| PubMed ID | 19526100 |
