Relevance of resistance levels to carbapenems and integron-borne blaIMP-1, blaIMP-7, blaIMP-10 and blaVIM-2 in clinical isolates of Pseudomonas aeruginosa. Journal of medical microbiology [J Med Microbiol] Journal article | | Title | Relevance of resistance levels to carbapenems and integron-borne blaIMP-1, blaIMP-7, blaIMP-10 and blaVIM-2 in clinical isolates of Pseudomonas aeruginosa. | | Author(s) | Zhao WH, Chen G, Ito R, Hu ZQ | | Institution | Showa University School of Medicine. | | Source | J Med Microbiol 2009 Jun 15. | | Abstract | The molecular detection and surveillance of the resistant genes harbored by Pseudomonas aeruginosa become more and more important, to assess and control their spread and colonization in hospitals, and to guide treatment of the infections. In this study, we analyzed the resistance mechanisms of carbapenem-resistant clinical isolates of P. aeruginosa and identified the associated integron-borne metallo-beta-lactamase (MBL) genes. Twenty-seven imipenem-resistant clinical isolates of P. aeruginosa were divided into three groups according to their resistance levels to carbapenems. Strains bearing bla(IMP-10) showed extremely high level resistance to imipenem with MICs of 512 to 2048 mug ml(-1). By comparison, strains bearing the bla(IMP-1), bla(IMP-7) and bla(VIM-2) showed the middle level of resistance with MICs of 32 to 256 mug ml(-1). The non-MBL-producing strains showed low level of resistance with MICs of 8 to 32 mug ml(-1). The same trend in resistance levels was also observed when resistance to other carbapenems such as meropenem and panipenem were determined. DNA sequencing showed that the MBL gene cassettes were carried by class 1 integrons. The bla(IMP-1), bla(IMP-7) and bla(IMP-10) gene cassettes were preceded by a hybrid P(ant) promoter, TGGACA-N(17)-TAAACT, and the bla(VIM-2) gene cassette was preceded by a weak P(ant) promoter, TGGACA-N(17)-TAAGCT. Most of the MBL genes were linked with one or two resistance genes encoding aminoglycoside-modifying enzymes, such as aac(6')Iae, aac(6')II, aacA7, aacC4, aadA1, aadA2 and aadA6, highlighting the multidrug-resistant properties of these clinical isolates. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19528141 |
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