Yoshida H, Sudo M, Todoroki K, Nohta H, Yamaguchi M
Highly selective and simple method for determination of polythiols based on liquid chromatography with postcolumn excimer fluorescence derivatization. [Journal Article, Research Support, Non-U.S. Gov't]
Anal Sci 2009 Jun; 25(6):829-32.
An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer fluorescence (440-520 nm). In this study, dimercaprol and dithiothreitol were used as model polythiols. This polythiol analysis method is simple; it is also highly selective and sensitive and yields good calibration curves.
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