| Title | Highly selective and simple method for determination of polythiols based on liquid chromatography with postcolumn excimer fluorescence derivatization. | | Author(s) | Yoshida H, Sudo M, Todoroki K, Nohta H, Yamaguchi M | | Institution | Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Johnan, Fukuoka 814-0180, Japan. | | Source | Anal Sci 2009 Jun; 25(6):829-32. | | Abstract | An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer fluorescence (440-520 nm). In this study, dimercaprol and dithiothreitol were used as model polythiols. This polythiol analysis method is simple; it is also highly selective and sensitive and yields good calibration curves. | | Language | eng | | Pub Type(s) | Journal Article
Research Support, Non-U.S. Gov't
| | PubMed ID | 19531896 |
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