Kim DJ, Seok SH, Baek MW, Lee HY, Juhn JH, Lee S, Yun M, Park JH
Highly expressed recombinant human follicle-stimulating hormone from Chinese hamster ovary cells grown in serum-free medium and its effect on induction of folliculogenesis and ovulation. [JOURNAL ARTICLE]
Fertil Steril 2009 Jun 15.
OBJECTIVE: To develop efficient Chinese hamster ovary (CHO) cells that express recombinant human FSH (rhFSH) in serum-free conditions and to investigate the effect of this newly synthesized rhFSH on folliculogenesis and ovulation.
DESIGN: Experimental study.
SETTING: Seoul National University, South Korea.
ANIMALS: Forty immature hypophysectomized rats and 40 androgen-sterilized mice.
INTERVENTION(S): A stable single CHO cell that expresses rhFSH at a high level was obtained by introducing the human chorionic gonadotropin (hCG) alpha-subunit and FSH beta-subunit genes. After purification processing, we investigated the effect of this newly synthesized rhFSH on folliculogenesis in hypophysectomized rats and ovulation in androgen-sterilized mice.
MAIN OUTCOME MEASURE(S): The ovary weight, uterine weight, number of follicles, and ovarian morphology were evaluated in immature hypophysectomized rats. The number of ovulated oocytes and ovarian morphology were examined in androgen-sterilized mice.
RESULT(S): After purification processing, we analyzed the new rhFSH using matrix-associated laser desorption ionization-time of flight and found that this new rhFSH increased both ovarian weight and uterine weight in hypophysectomized rats and induced ovulation in androgen-sterilized mice.
CONCLUSION(S): This newly synthesized rhFSH might be safely used in anovulatory infertile woman as well as in ovulation induction protocols for subfertile women.
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