On-line solid-phase extraction combined with liquid chromatography-tandem mass spectrometry for high throughput analysis of 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid in urine. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | On-line solid-phase extraction combined with liquid chromatography-tandem mass spectrometry for high throughput analysis of 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid in urine. | | Author(s) | Fernández MD, Wille SM, Samyn N, Wood M, López-Rivadulla M, De Boeck G | | Institution | Federal Public Service Justice, National Institute of Criminalistics and Criminology, Chaussee de Vilvorde 100, 1120 Brussels, Belgium. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2009 May 21. | | Abstract | A simple, rapid and highly sensitive method for the analysis of THC-COOH in urine, using automated on-line solid-phase extraction (SPE) combined with liquid chromatography (LC)-mass spectrometry (MS/MS), is developed and fully validated according to international guidelines. Chromatographic separation was achieved on an Atlantis dC(18) column with an isocratical gradient, ensuring the elution of THC-COOH within 4.1min. The total process time was 6min and 500muL of sample was required. SPE using C(8) cartridges was highly effective, reproducible and led to significant decreases in the interferences present in the matrix. The method showed an excellent intra- and inter-assay precision (relative standard deviation (RSD) <7% and bias <13%) for four external quality control (QC) samples and three 'in house' QCs. Responses were linear over the investigated range (r(2)>0.99, 5-200mug/L). Limits of quantification (LOQ) and detection (LOD) were determined to be 5mug/L and 0.25mug/L, respectively. Furthermore, the analyte and the processed samples were demonstrated to be stable. Moreover, no carryover was observed after the analysis of high concentrated urine samples (5000mug/L THC-COOH)). The method was subsequently applied to authentic samples previously screened by a routine immunoassay method. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19535306 |
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