| Title | Synthesis of dinitrophenyl-l-Pro-N-hydroxysuccinimide ester and four new variants of Sanger's reagent having chiral amines and their application for enantioresolution of mexiletine using reversed-phase high-performance liquid chromatography. | | Author(s) | Bhushan R, Tanwar S | | Institution | Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India. | | Source | J Chromatogr A 2009 Jun 6. | | Abstract | Four chiral derivatizing reagents (CDRs) having enantiomerically pure amines and two CDRs namely, [N-succinimidyl-(S)-2-(6-methoxynaphth-2-yl)propionate], and [dinitrophenyl-l-Pro-N-hydroxysuccinimide ester, DNP-l-Pro-SU] were synthesized and were used to prepare diastereomers of (R,S)-mexiletine (MEX); these were separated by reversed-phase high-performance liquid chromatography (RP-HPLC). The method was validated for linearity, accuracy, limit of detection (LOD) and limit of quantification (LOQ). | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19552908 |
|
