| Title | Cellular models for the screening and development of anti-hepatitis C virus agents. | | Author(s) | Gondeau C, Pichard-Garcia L, Maurel P | | Institution | Inserm, U632, Montpellier, F-34293 France; Université Montpellier1, UMR-S632, Montpellier, F-34293 France; CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint Eloi, Montpellier, F-34295 France. | | Source | Pharmacol Ther 2009 Jun 22. | | Abstract | Investigations on the biology of hepatitis C virus (HCV) have been hampered by the lack of small animal models. Efforts have therefore been directed to designing practical and robust cellular models of human origin able to support HCV replication and production in a reproducible, reliable and consistent manner. Many different models based on different forms of virions and hepatoma or other cell types have been described including virus-like particles, pseudotyped particles, subgenomic and full length replicons, virion productive replicons, immortalised hepatocytes, fetal and adult primary human hepatocytes. This review focuses on these different cellular models, their advantages and disadvantages at the biological and experimental levels, and their respective use for evaluating the effect of antiviral molecules on different steps of HCV biology including virus entry, replication, particles generation and excretion, as well as on the modulation by the virus of the host cell response to infection. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19555718 |
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