| Title | Flavonoids from Inula Britannica L. inhibit injury-induced neointimal formation by suppressing oxidative-stress generation. | | Author(s) | Zhang HB, Wen JK, Wang YY, Zheng B, Han M | | Institution | Department of Biochemistry and Molecular Biology, Institute of Basic Medicine, Hebei Medical University, No. 361, Zhongshan East Road, Shijiazhuang 050017, P. R. China; Biological Science and Engineering Institute, Hebei University of Economics and Business, Shijiazhuang 050061, P.R. China. | | Source | J Ethnopharmacol 2009 Jun 23. | | Abstract | AIM OF THE STUDY: We aimed to investigate whether and how the total flavonoid extracts (TFE) from Inula Britannica L. block neointimal hyperplasia induced by balloon injury in rats.
MATERIALS AND METHODS: Rats were administered orally at TFE doses of 12.5, 25 and 50mg/kg/d by gastric gavage from 3 days before balloon injury to 14 days after the injury, respectively. The ratio of intima (I) to media (M) thickness (I/M) in carotid arteries was examined by morphological analyses. The MDA content and SOD activity in plasma were measured, respectively. The O(2)(-)production in vascular tissues was detected in situ. The expression of p47(phox) in carotid arteries was analyzed by Western blot analysis and immunohistochemistry, respectively.
RESULTS: The rats treated with TFE 50mg/kg/d showed a reduction in neointimal hyperplasia, and the ratio of I/M of balloon injured-carotid arteries was significantly reduced by over 70% after TFE treatment, compared with the injured group. The inhibitory effect of TFE on neointimal hyperplasia was almost consistent with that of atorvastatin, a positive control. The plasma SOD activity was obviously increased by TFE treatment (P < 0.01), while plasma MDA production was markedly decreased by TFE treatment (P < 0.05). On day 14 after balloon injury, the carotid arteries showed an increase in O(2)(-) production that was most evident in the neointimal and medial layer of the vessel. Thus, TFE significantly inhibited injury-induced O(2)(-) production and p47(phox) expression in carotid arteries.
CONCLUSION: Our results suggest that TFE inhibit the neointimal hyperplasia after balloon injury, at least partly, by suppressing oxidative-stress generation. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19559080 |
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