| Title | Effects of isopulegol on pentylenetetrazol-induced convulsions in mice: Possible involvement of GABAergic system and antioxidant activity. | | Author(s) | Silva MI, Silva MA, de Aquino Neto MR, Moura BA, de Sousa HL, de Lavor EP, de Vasconcelos PF, Macêdo DS, de Sousa DP, Vasconcelos SM, de Sousa FC | | Institution | Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Address: Rua Cel. Nunes de Melo 1127, CEP: 60430-270, Fortaleza, Brazil. | | Source | Fitoterapia 2009 Jun 24. | | Abstract | The present study investigated the effects of isopulegol, a monoterpene alcohol, in PTZ-induced convulsions and verified possible involved mechanisms. Saline, isopulegol or diazepam were intraperitonealy injected 30 minutes before PTZ. The latency for development of convulsions and mortality, as well as the mortality protection percentage were recorded. For investigating the involvement of GABAergic system, flumazenil was utilized. The activity of antioxidant enzymes (catalase and reduced glutathione) and the lipid peroxidation levels were measured in brain hippocampus. Similarly to diazepam, isopulegol significantly prolonged the latency for convulsions and mortality of mice. All animals were protected against mortality at higher dose of isopulegol. Flumazenil pretreatment decreased the prolongation of seizure latency induced by both diazepam and isopulegol, although was not able to reverse the latency and protection percent for mortality. Isopulegol also significantly prevented PTZ-induced increase in lipid peroxidation, preserved catalase activity in normal levels, and prevented the PTZ-induced loss of GSH in hippocampus of mice. These results suggest that the anticonvulsant and bioprotective effects of isopulegol against PTZ-induced convulsions are possibly related to positive modulation of benzodiazepine-sensitive GABA(A) receptors and to antioxidant properties. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19559770 |
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