Bobin-Dubigeon C, Amiand MB, Herrenknecht C, Bard JM
Development and validation of an improved liquid chromatography-mass spectrometry method for the determination of pemetrexed in human plasma. [JOURNAL ARTICLE]
J Chromatogr B Analyt Technol Biomed Life Sci 2009 Jun 21.
An improved liquid chromatography-mass spectrometry method for the determination of pemetrexed in human plasma was developed and validated using a simple quadrupole LC-MS and a new SPE cartridge (Plexa((R)) Bond Elut). The analysis was achieved with a C18 analytical column using a mobile phase consisting of formic acid/acetonitrile and isocratic flow for 7min. The linear ranges (r(2)>0.99) were found from 5 to 5000ng/mL. The lower limit of detection was 2.5ng/mL. Within-day and between-day precisions were less than 7.2% and inaccuracy did not exceed 2.8%. This new method is suitable to support pharmacokinetic studies and drug monitoring.
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