Development and validation of an improved liquid chromatography-mass spectrometry method for the determination of pemetrexed in human plasma. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] Journal article | | Title | Development and validation of an improved liquid chromatography-mass spectrometry method for the determination of pemetrexed in human plasma. | | Author(s) | Bobin-Dubigeon C, Amiand MB, Herrenknecht C, Bard JM | | Institution | Department of Oncology Biology, CLCC René Gauducheau, Bd J Monod 44805 Nantes Saint Herblain Cedex, France; Université de Nantes Faculté de Pharmacie, EA 2160 MMS, 1 rue Gaston Veil, 44000 Nantes, France. | | Source | J Chromatogr B Analyt Technol Biomed Life Sci 2009 Jun 21. | | Abstract | An improved liquid chromatography-mass spectrometry method for the determination of pemetrexed in human plasma was developed and validated using a simple quadrupole LC-MS and a new SPE cartridge (Plexa((R)) Bond Elut). The analysis was achieved with a C18 analytical column using a mobile phase consisting of formic acid/acetonitrile and isocratic flow for 7min. The linear ranges (r(2)>0.99) were found from 5 to 5000ng/mL. The lower limit of detection was 2.5ng/mL. Within-day and between-day precisions were less than 7.2% and inaccuracy did not exceed 2.8%. This new method is suitable to support pharmacokinetic studies and drug monitoring. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19560408 |
|
|
| |
Advertise on this site.
| | |
|
