Lei H, Gui L, Xiao R
The effect of anticoagulants on the quality and biological efficacy of platelet-rich plasma. [JOURNAL ARTICLE]
Clin Biochem 2009 Jun 25.
OBJECTIVES: This study investigated the effect of anticoagulants on platelet-rich plasma (PRP) quality to determine the appropriate anticoagulants for PRP production.
DESIGN AND METHODS: This study was carried out at Plastic Surgery Hospital of Peking Union Medical College. The microstructure of platelets collected with heparin, citrate, acid-citrate-dextrose (ACD) and citrate-theophylline-adenosine-dipyridamole (CTAD) was observed. The extent of spontaneous activation of platelets was detected by measuring sP-selectin in plasma. The amount of TGF-beta1 released from PRP and the effect of PRP on cell proliferation were also studied.
RESULTS: ACD and CTAD were superior to heparin and citrate in maintaining the integrity of platelet structures and preventing the platelet spontaneous activation. ACD-PRP and CTAD-PRP released more TGF-beta1 and significantly enhanced the proliferation of human marrow stromal cells compared to heparin-PRP and citrate-PRP.
CONCLUSIONS: The PRP quality was closely related to the type of anticoagulants. ACD and CTAD are appropriate anticoagulants for PRP production.
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