| Title | The effect of anticoagulants on the quality and biological efficacy of platelet-rich plasma. | | Author(s) | Lei H, Gui L, Xiao R | | Institution | Department six of Plastic Surgery Hospital, Peking Union Medical College, China Academy of Medical Sciences, No.33 BaDaChu Road, Shijingshan District, Beijing , 100144, China. | | Source | Clin Biochem 2009 Jun 25. | | Abstract | OBJECTIVES: This study investigated the effect of anticoagulants on platelet-rich plasma (PRP) quality to determine the appropriate anticoagulants for PRP production.
DESIGN AND METHODS: This study was carried out at Plastic Surgery Hospital of Peking Union Medical College. The microstructure of platelets collected with heparin, citrate, acid-citrate-dextrose (ACD) and citrate-theophylline-adenosine-dipyridamole (CTAD) was observed. The extent of spontaneous activation of platelets was detected by measuring sP-selectin in plasma. The amount of TGF-beta1 released from PRP and the effect of PRP on cell proliferation were also studied.
RESULTS: ACD and CTAD were superior to heparin and citrate in maintaining the integrity of platelet structures and preventing the platelet spontaneous activation. ACD-PRP and CTAD-PRP released more TGF-beta1 and significantly enhanced the proliferation of human marrow stromal cells compared to heparin-PRP and citrate-PRP.
CONCLUSIONS: The PRP quality was closely related to the type of anticoagulants. ACD and CTAD are appropriate anticoagulants for PRP production. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19560449 |
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