Mali B, Armbruster D, Serediak E, Ottenbreit T
Comparison of Immunoturbidimetric and Immunonephelometric Assays for Specific Proteins. [JOURNAL ARTICLE]
Clin Biochem 2009 Jun 25.
Immunoturbidimetric assays for specific proteins are available on "open system" clinical chemistry analyzers. The analytical performance of nine immunoturbidimetric specific protein assays (C3, C4, CRP, Haptoglobin, IgA, IgG, IgM, RF, Transferrin) were compared to immunonephelometry. Testing was performed on the Abbott ARCHITECT c8000 and the Dade Behring BNII nephelometer and evaluated for precision, linearity, limit of detection, prozone phenomenon, method comparison, workflow, and proficiency testing survey comparison. Immunoturbidimetric assays performance was satisfactory for total precision, linearity, limit of detection and the prozone effect was not observed. Method comparison was acceptable for the immunoglobulins, CRP and transferrin but less favorable for the other assays, likely due to methodology and antibody specificity differences. Immunourbidimetric specific protein assays allow for efficient test consolidation on a general purpose clinical chemistry analyzer.
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