| Title | Tissue-engineered thyroid cell sheet rescued hypothyroidism in rat models after receiving total thyroidectomy comparing with non-transplantation models. | | Author(s) | Arauchi A, Shimizu T, Yamato M, Obara T, Okano T | | Institution | Tokyo Women's Medical University, TWIns, Institute of Advanced Biomedical Engineering and Science, Tokyo, Japan; arauchi@abmes.twmu.ac.jp. | | Source | Tissue Eng Part A 2009 Jun 30. | | Abstract | For hormonal deficiency due to endocrine organsdiseases, continuous oral hormone administration is indispensable in order to supplement the shortage of hormones. In this study, as a more effective therapy, we have tried to reconstruct the three-dimensional thyroid tissue by novel tissue engineering approach 'cell sheet technology'. The cell suspension obtained from rat thyroid gland was cultured on temperature-responsive culture dishes, from which confluent cells detach as a cell sheet simply by reducing temperature without any enzymatic treatment. The 8-week-old Lewis rats were exposed to total thyroidectomy as hypothyroidism models and received thyroid cell sheet transplantation one week following total thyroidectomy. Serum levels of freeT3 and freeT4 significantly decreased one week after total thyroidectomy. On the other hand, transplantation of the thyroid cell sheets was able to restore the thyroid function one week after the cell sheet transplantation and improvement was maintained for 4 weeks. Moreover, morphological analyses showed typical thyroid follicle organization and anti-Thyroid-Transcription-Factor-1 antibody staining demonstrated the presence of follicle epithelial cells. The presence of functional microvessels was also detected within the engineered thyroid tissues. In conclusion, our results indicate that thyroid cell sheets transplanted in a model of total thyroidectomy can reorganize histologically to resemble a typical thyroid gland and restore thyroid function in vivo. In this study, we are the first to confirm that engineered thyroid tissue can repair hypothyroidism models in rats. Therefore, cell sheet transplantation of endocrine organs may be suitable for the therapy of hormonal deficiency. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19566441 |
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