| Title | Fast liquid chromatography separation and multiple-reaction monitoring mass spectrometric detection of neurotransmitters. | | Author(s) | Hammad LA, Neely M, Bridge B, Mechref Y | | Institution | METACyt Biochemical Analysis Center, Department of Chemistry, Indiana University, Bloomington, USA. Fax: +1-812-8558300. | | Source | J Sep Sci 2009 Jun 30. | | Abstract | We describe here the fast LC-MS/MS separation of a mixture of neurotransmitters consisting of dopamine, epinephrine, norepinephrine, 3,4-dihydroxybenzylamine (DHBA), salsolinol, serotonin, and gamma-aminobutyric acid (GABA). The new UltiMate(R) 3000 Rapid Separation system (RSLC) was successfully coupled to the 4000 QTRAP mass spectrometer operating in multiple-reaction monitoring (MRM) mode. The separation was attained using a 100 mm length, 2.2 mum particle size Acclaim column at a flow rate of 0.5 mL/min. The column back pressure was 350 bar, while the total run time including column re-equilibration was 5.2 min. The peak resolution was minimally affected by the fast separation. The RSLC-MRM separation was found to have a precision range based on peak area for 50 replicate runs of 2-5% CV for all analytes, and the reproducibility of the retention time for all analytes was found to range from 0-2% CV. The described method represents an almost seven times shorter analysis time of neurotransmitters using LC/MRM which is very useful in screening large quantities of biological samples for various neurotransmitters. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19569096 |
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