Highly cross-linked polymeric capillary monoliths for the separation of low, medium, and high molecular weight analytes. Journal of separation science [J Sep Sci] Journal article | | Title | Highly cross-linked polymeric capillary monoliths for the separation of low, medium, and high molecular weight analytes. | | Author(s) | Lubbad SH, Buchmeiser MR | | Institution | Leibniz-Institut für Oberflächenmodifizierung, Leipzig, Germany. Fax: +49-0-3412352584. | | Source | J Sep Sci 2009 Jun 30. | | Abstract | Highly rigid capillary monoliths with low swelling propensity were prepared within the confines of 200 mum ID fused silica capillaries via thermally induced free radical polymerization of tetrakis(4-vinylbenzyl)silane (TVBS) in the presence of 1-dodecanol and toluene. alpha,alpha'-Azobisisobutyronitrile (AIBN) was used as initiator. The resulting monoliths were optimized for the separation of low, medium, and high molecular weight analytes. The microstructure and porosity of the monoliths prepared were studied by SEM and inverse size-exclusion chromatography (ISEC). The porosity of the monolithic supports was tuned by varying the amount of initiator (i. e. AIBN) between 0.5 and 2 wt%. All monoliths were tested for a series of low molecular weight compounds including alkylbenzenes, amines, carboxylic acids, phenols, carbonyl compounds, and beta-blockers, as well as for the separation of medium molecular weight analytes such as peptides and high-molecular weight analytes such as proteins. Due to the microporous structure, the novel monoliths displayed high efficiency and performance particularly in the separation of low molecular weight analytes. Relevant chromatographic parameters including permeability, swelling propensity, and height equivalents to theoretical plates were determined. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19569097 |
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