| Title | Regulator of G protein signalling 16 (RGS16) is a target for a porcine circovirus type-2 protein. | | Author(s) | Timmusk S, Merlot E, Lövgren T, Järvekülg L, Berg M, Fossum C | | Institution | Tallinn University of Technology; | | Source | J Gen Virol 2009 Jul 1. | | Abstract | Interaction studies have suggested that the non-structural protein encoded by open reading frame 3 (ORF3) of porcine circovirus type 2 (PCV2) binds specifically to a regulator of G protein signalling (RGS) related to human RGS16. The full-length clone of RGS16 was generated from porcine cells and sequence analysis revealed a close relationship to huRGS16 and muRGS16. In vitro pull down experiments verified an interaction between poRGS16 and ORF3 from PCV2. Using GST-linked ORF3 proteins from two different genogroups of PCV2 and from porcine circovirus type 1 (PCV1) in the pull down experiments, indicated differences in their ability to bind poRGS16. Quantitative RT-PCR demonstrated that the expression of mRNA for poRGS16 could be induced by a number of cell activators including mitogens (LPS and PHA), interferon inducers (ODN 2216 and poly I:C) and the neurotransmitter norepinephrine (NE). Immunofluorescence labelling confirmed the induced expression of poRGS16 at the protein level and suggested that the PCV2 ORF3 protein co-localized with poRGS16 in LPS activated poPBMC. Furthermore, poRGS16 appeared to participate in the translocation of the ORF3 protein into the cell nucleus, suggesting that the observed interaction may play an important role in the infection biology of porcine circovirus. | | Language | ENG | | Pub Type(s) | JOURNAL ARTICLE
| | PubMed ID | 19570954 |
|
